Detection Of Seed-Borne Infection Of Tomato (Solanum Lycopersicuml.) Seeds By Agar Plate And Blotter Methods

  • E N.K Sowley
  • J Kodua


Tomato is a popular, widely cultivated and consumed vegetable crop in the world, but its production is hampered by diseases, some of which are seed-borne. This study sought to detect seed-borne fungi in tomato seeds used by farmers in the,Tolon-Kurnbungu District of Northern Region of Ghana_ Twenty seed samples made up of two from commercial seed retailers and 18 from farmers in nine communities were used for the detection of the seed-borne mycoflora, by the agar plate and blotter methods in the Plant Pathology laboratory of the Savannah Agricultural Research Institute (SARI). Four fungal species namely Aspergillus flavus (Link), A. niger (van Tieghem), Penicilium spp. and Rhizopus stolonifer (Ehrenb. :Fr) Vuil., were isolated and identified from seeds stored by farmers. The Agar plate method was more sensitive than the blotter method in detecting the seed-borne fungi. Aspergillus flavus had the highest occurrence in both sterilised and unsterilised seeds while Penicillium had the least. For example, the occurrence of A. flavus in seeds tested by the blotter method for sterilised and unsterilised seeds were 56.7% and 56.1%, respectively and 3.2% and 3.5%, respectively for Penicillium. Generally, the incidence of seed-borne fungi in samples offarmer-saved seeds was higher than the commercial seeds. All the seed samples tested had a germination percentage above 80%, except sample GYA from Gareshegu) which had 61.5% germination. Due to the high popularity of the farmer-saved seeds, a system should be put in place to frequently check the health status of these farmer-saved seeds, to prevent the use of infected seeds in tomato production, which could have adverse consequences on the yield